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Hypo-osmotic stress does not affect intracellular cAMP response after βAR stimulation. Normalized emission intensity ratio (eCFP/FRET) changes of the cAMP sensor ICUE3 was measured in A10 cells treated with 5 μm isoprenaline under 300 and 150 mosm conditions. Bleach control cells were transfected with ICUE3 but not treated with isoprenaline. Six to seven cells were measured in each group.
