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Primer sequences
fw indicates forward, and rv indicates reverse.
| No. | Construct | Name | 5′ |
|---|---|---|---|
| 1 | His:HA:NEDD8 | HAN8 NdeI fw | AATATCATATGTATCCATACGATGTTCCAG |
| ATTATGCTGTCGGTGGAGGAGGTGGTACT | |||
| ATGATTAAGGTG | |||
| 2 | His:HA:NEDD8 | N8 EcoRI rv | ATGAATTCTTAACCACCCCTAAGGGCAAG |
| 3 | FLAG:AXR1 | FLAG-AXR1 NdeI fw | ATCCATATGGACTACAAAGACGATGACGAC |
| AAGGGTATGCAAGCAGTAAAAAGATC | |||
| 4 | FLAG:AXR1 | AXR1 NotI rv | ATGCGGCCGCCTACAATTTCAATAACTGAG |
| 5 | ECR1:His | ECR1 NcoI fw | ATCCATGGCTGATCTCGATGTTC |
| 6 | ECR1:His | ECR1 XhoI rv | ATCTCGAGTGCTCCAATGGCTGTATC |
