FIGURE 2.

The ectopic expression of paxillin in HeLa cells causes them to lose their transformed phenotype. A and B, two sets of HeLa cells were transfected with vector alone, GFP-tagged wild-type paxillin (Pxn WT), or V5-tagged Cat-1 KK-RA. One set (A) was lysed and then immunoblotted with GFP, V5, and actin antibodies. Soft agar assays were performed (B) on the other set of cells. The number of colonies that formed for each condition was determined. All of the experiments shown were performed three separate times, each yielding similar results. The data shown in B represent mean ± S.D. Student's t tests were performed. *, p < 0.05.