FIGURE 3.

The inability of HeLa cells depleted of Cat-1 to grow under anchorage-independent conditions is restored by knocking down paxillin expression. A and B, two sets of HeLa cells were transfected with the indicated combinations of Cont. siRNA, Cat-1 siRNA, and Pxn siRNA. One set (A) was lysed and then immunoblotted with Cat-1 and paxillin antibodies. Soft agar assays were performed (B) on the other set of cells. The number of colonies that formed for each condition was determined. All of the experiments shown were performed three separate times, each yielding similar results. The data shown in B represent mean ± S.D. Student's t tests were performed. *, p < 0.05.