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. 2017 Jan 18;292(9):3947–3957. doi: 10.1074/jbc.M116.769190

FIGURE 5.

FIGURE 5.

The levels of Akt phosphorylation are dependent on Cat-1 and paxillin expression. A, HeLa cells transfected with the indicated combinations of Cont. siRNA, Cat-1 siRNA, and Pxn siRNA were lysed and then immunoblotted with phospho-Akt (Ser-473), Akt, phospho-ERK, and ERK antibodies. B, HeLa cells transfected with either control siRNA or paxillin siRNA were lysed and then immunoblotted with phospho-Akt (Ser-473) and Akt antibodies. C, HeLa cells transfected with either the vector-alone (Vector) or GFP-tagged wild-type paxillin (Pxn WT) were lysed and then immunoblotted with phospho-AKT (Ser-473) and Akt antibodies. D, HeLa cells transfected with either Cont. siRNA or Cat-1 siRNA were lysed and then immunoblotted with phospho-Pxn (Tyr-118) and Pxn antibodies. E, HeLa cells transfected with either Cont. siRNA or Cat-1 siRNA were grown in a monolayer (Attached) or placed in suspension (Suspended) before being lysed and immunoblotted with phospho-Pxn (Tyr-118), Pxn, phospho-Akt (Ser-473), and Akt antibodies. All of the experiments shown were performed three separate times, each yielding similar results. The relative changes in ERK and AKT phosphorylation for each condition in A and B was determined.