FIGURE 6.

Cat-1 regulates the phosphorylation of both Akt1 and Akt2. A, HeLa cells transfected with either Cont. siRNA or Cat-1 siRNA were lysed and then immunoblotted with Cat-1, Akt1, and Akt2 antibodies. B, the same cell lysates generated in A were further immunoblotted with antibodies that specifically detect phospho-Akt1 (Ser-473) and Akt1 (left panel) and phospho-Akt2 (Ser-473) and Akt2 (right panel). All of the experiments shown were performed three separate times, each yielding similar results. The relative changes in Akt1 and Akt2 phosphorylation for each condition in B was determined.