FIGURE 7.

Akt1 and Akt2 contribute to the anchorage-independent growth of HeLa cells. A and B, two sets of HeLa cells were transfected with the indicated combinations of Cat-1 siRNA, Akt1 siRNA, and Akt2 siRNA or treated with Akt inhibitor VIII (2 μm). One set (A) was lysed and then immunoblotted with Akt1 and Akt2 antibodies. Soft agar assays were performed (B) on the other set of cells. The number of colonies that formed for each condition was determined. All of the experiments were performed three separate times, each yielding similar results. The data shown in B represent mean ± S.D. Student's t tests were performed. *, p < 0.05; **, p < 0.01.