FIGURE 8.

The block in HeLa cell transformation caused by the depletion of Cat-1 is rescued by the ectopic expression of activated forms of AKT. A and B, two sets of HeLa cells stably expressing the indicated combinations of the vector alone and constitutively active forms of Akt1 (Akt1 DD) and Akt2 (Akt2 DD) were transfected with either Cont. siRNA or Cat-1 siRNA. One set (A) was lysed and then immunoblotted with Cat-1 and HA antibodies. The vertical lines represent a portion of the blots that were cropped. Soft agar assays were performed (B) on the other set of cells. The number of colonies that formed for each condition was determined. All of the experiments shown were performed three separate times, each yielding similar results. The data shown in B represent mean ± S.D. Student's t tests were performed. *, p < 0.05; **, p < 0.01.