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. 2017 Mar 7;7:44213. doi: 10.1038/srep44213

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Fluoresence images of F-actin (green) and nucleus (blue) stained cells on (a) the as-received, (b) eCG, (c) eFG, and (d) eUFG substrates after 3 days. (e) The average cell adhesion area on each substrate by measuring the region of F-actin fluorescence using image J software (^***p < 0.001 and ^**p < 0.01). The fluorescence images of F-actin were used false color from red to green, and merged with the nucleus fluorescence images. (f) Proliferation rate of the pre-osteoblast cells with a reference of the as-received CP-Ti substrate (^***p < 0.001, ^**p < 0.01, and ^*p < 0.05).