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. 2017 Mar 7;7:44140. doi: 10.1038/srep44140

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(A) HE staining showed that suspension of ECM microfilament was ivory and ropiness; (B and C) The positive of staining showed fibriform of microfilament was disorderly mixed with cell debris; (D and E) The positive of Safranin O and TB staining showed purified microfilaments of growth plate richly contained GAG. (F) The positive staining showed ECM microfilaments contained collagen II. (G and H) Hoechst 33258 staining showed cell debris of growth plate in suspension before centrifuged with different velocity; (I) No cells or cell fragments were present in scaffold after different velocity of centrifugalization.