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. 2017 Mar 7;7:44252. doi: 10.1038/srep44252

Figure 5. Effect of neoagarotetraose on the activation of MAPK pathway in RAW264.7 cells.

Figure 5

(A) After pretreated with neoagarotetraose (125, 250, 500 μg/ml) for 2 h, RAW264.7 cells were exposed to 100 ng LPS for 1 h. Then the expression levels of p38MAPK and phosphorylated p38MAPK were detected by western blot, respectively. Blots were also probed for β-actin as loading controls. The result shown is a representative of three separate experiments with similar results. (B) Quantification of immunoblot for the ratio of p38MAPK or phosphorylated p38MAPK to β-actin. The ratio for non-treated control cells was assigned a value of 1.0 and the data presented as mean ± SD (n = 3). Significance: ##P < 0.01 vs. normal control; **P < 0.01 vs. LPS treated control. (C) After treatment, the expression levels of ERK1/2 and phosphorylated ERK1/2 were detected by western blot, respectively. (D) Quantification of immunoblot for the ratio of total ERK1/2 or phosphorylated ERK1/2 to β-actin. The ratio for non-treated normal control cells was assigned a value of 1.0 and the data presented as mean ± SD (n = 3). Significance: #P < 0.05 vs. normal control; *P < 0.05 vs. LPS treated control.