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. 2017 Mar 7;7:43733. doi: 10.1038/srep43733

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The fertilization rate of eEF1A1b^+/− XY fish was significantly lower than that of eEF1A1b^+/+ XY fish (A). Sperm of eEF1A1b^+/+ XY fish were with normal flagella, while most sperm of eEF1A1b^+/− XY fish were flagella-less, and the ratio of sperm with morphological abnormalities to the total sperm was higher in eEF1A1b^+/− XY fish than in eEF1A1b^+/+ XY fish (B-D). The beat frequency of sperm flagella was higher in eEF1A1b^+/+ XY fish than in eEF1A1b^+/− XY fish (E). And the ratio of forward motility sperm was higher in eEF1A1b^+/+ XY fish than in eEF1A1b^+/− XY fish (F-H). The red line indicates the trajectories of sperm. Results were expressed as mean ± SD. Different letters indicate statistical differences at P < 0.05 as determined by one-way ANOVA followed by post hoc test.