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. 2017 Mar 7;7:43733. doi: 10.1038/srep43733

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Copyright © 2017, The Author(s)

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(a) Confirmation of eEF1A1b transgene insertion by genomic PCR. Lane 1 and 14, DNA marker; Lane 2-7, XY fish carrying eEF1A1b-transgene; Lane 8-13, XY control fish; Lane 15, empty plasmid as template. (b) A, the GFP signals in the testis of eEF1A1b^+/−/TG XY fish in a dark field; Arrowheads indicate the positive signal. B, bright field; C-H, IHC analyses of Vasa, PH3 and Cyp11b2 expression in the testis of eEF1A1b^+/−/TG and eEF1A1b^+/− XY fish; SG, spermatogonia; SC, spermatocytes; ST, spermatids. (c) Expression of eEF1A1b, cyp11b2, vasa, suz12, usp26 and spo11 in the eEF1A1b^+/−/TG, eEF1A1b^+/− and eEF1A1b^+/+ XY fish by real-time PCR. (d) Serum 11-KT levels of the eEF1A1b^+/−/TG, eEF1A1b^+/− and eEF1A1b^+/+ XY fish. (e) GSI of eEF1A1b^+/−/TG, eEF1A1b^+/− and eEF1A1b^+/+ XY fish. Results were expressed as mean ± SD. Different letters indicate statistical differences at P < 0.05 as determined by one-way ANOVA followed by post hoc test.