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. 2017 Mar 7;7:43094. doi: 10.1038/srep43094

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Lateral view of 3D image of the canal network in the endplate after aging obtained using PPCST with the cranial and caudal endplates separately displayed. The endplate was divided into three different regions, anterior, center and posterior. (B) Representative image of the corresponding histology sections of the cranial and caudal endplates for various ages. (C) Quantitative analysis of the morphological parameters in the cranial and caudal endplates in three regions after aging. Scale bar = 1 mm; A = anterior; C = center; P = posterior. Data are the means ± S.D., n = 8 mice in each group. In panel (B), a indicates a significant difference between the posterior region and the center region, and b indicates a significant difference between the posterior region and the anterior region (p < 0.05, determined by two-way repeated measures ANOVA with Bonferroni’s post hoc test).