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. 2004 Dec;24(24):10515–10528. doi: 10.1128/MCB.24.24.10515-10528.2004

FIG. 10.

FIG. 10.

RasGRP3 is upregulated in flt-1−/−blood vessels. (A) Northern blot analysis was performed on RNA isolated from flt-1−/− cultures (lane 3). RNA was also isolated from Rasgrp3+/+ and Rasgrp3gt/gt cultures treated with PMA (lanes 2 and 5) or left untreated (lanes 1 and 4). The blot was probed for RasGRP3 and then reprobed for the vascular marker PECAM. (B) Graph showing relative levels of RasGRP3 RNA in ES cell cultures. RasGRP3 RNA levels were normalized to the amount of PECAM RNA.