Figure 6.

Modification of the iMT chemical combinations enables lineage-specific trans-differentiation. (A) Detection of ANF-GFP signal on day 6 upon 6OTCFW treatment. (B) FACS analysis on Day 12 suggests high efficiency of embryonic cardiac trans-differentiation from MEFs by 6OTCFW treatment. (C) Transplantation of 6OTCFW-treated MEFs into the myocardial infarction regions reveals integration of the trans-differentiated cardiac myocytes with the cardiac tissues. Some transplanted cells (green fluorescence by ANF-GFP) also express cardiac-specific marker Troponin (red fluorescence by immunostaining). (D) Modified iMT culture condition induces efficient cardiac myocytic trans-differentiation from MEFs. (E) Gene expression analysis reveals cardiac-specific gene activation after stepwise chemical treatment (6TCF 4 Days + SOCFV 16 Days). Expression value is in Log₂ scale. MEFs were partially removed by 30 min of settling in tissue culture plate before analysis. (F) Modified iMT culture condition induces glial program from MEFs. (G) Gene expression analysis reveals neuronal or glial gene activation after stepwise chemical treatment (6TCF 6 Days + 8CFV 16 Days). Expression value is in Log₂ scale. (H) Modified iMT culture condition induces efficient adipocytic trans-differentiation from MEFs. (I) Gene expression analysis reveals adipocytic-specific gene activation after stepwise chemical treatment (6TCF 6 Days + 6TF 12 Days). Expression value is in Log₂ scale. MEFs were partially removed by 30 min of settling in tissue culture plate before analysis. (J) A diagram showing the roadmap for induced multi-lineage trans-differentiation by chemical combinations.