Skip to main content
. 2017 Feb 28;27(3):416–439. doi: 10.1038/cr.2017.26

Figure 2.

Figure 2

QKI5 regulates pri-124-1 processing during erythropoiesis. (A) q-PCR of endogenous pri-124-1 and miR-124 in K562 cells after 48-h transfection with individual RBP siRNAs. RBP candidates with opposite effects on pri-124-1 and miR-124 are indicated in red and blue, respectively. (B) A schematic representation of RBPs classified as either repressing (negative RBPs) or promoting (positive RBPs) pri-124-1 processing. The right panel shows immunoblot results of selected RBPs in both K562 cells and HPCs in E culture. (C) q-PCR analysis of pri-124-1 and miR-124 in K562 cells transfected with pCMV6-QKI5 or si-QKI5 for 48 h. Lower panel: immunoblot showing the levels of QKI5 (or Flag-QKI5) in pCMV6-QKI5- and si-QKI5-transfected K562 cells. Two sets of primers (#1 and #2, sequences are shown in Supplementary information, Table S4) were used to amplify pri-124-1. (D) q-PCR analysis of pri-124-1 and miR-124 in HPCs transduced with lenti-si-QKI5 or lenti-GFP in E culture. Lower panel: immunoblot of QKI5 in HPCs transduced with lenti-si-QKI5 or lenti-GFP in E culture. Error bars reflect SEM from three biological replicates if not stated otherwise. Significance was determined by t-test with *P< 0.05; **P< 0.01; ***P< 0.001.