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. 2017 Jan 13;27(3):352–372. doi: 10.1038/cr.2017.8

Figure 9.

Figure 9

CD146 as a target for atherosclerosis therapy. (A-G) Preventive targeting of CD146 with antibody reduces atherosclerosis. ApoE−/− mice were preventively injected with mIgG or AA98 when they began a Western diet (eight mice per group). All the quantifications were performed using at least 15 sections per mouse. (A) Intervention dosing regimen of CD146-targeted therapy using anti-CD146 AA98 or control IgG in high fat diet (HFD)-induced atherosclerosis. (B) Lesion area of plaques of the aortic roots of each group of mice, presented for each group across the 400 μm of the aortic root (n = 8). (C) Quantification of lesion area of aortic plaques isolated from ApoE−/− mice. (D) The distribution of early, moderate and advanced plaques based on histological staging of the atherosclerotic lesions. (E) Quantification of immunostained aortic plaques for macrophages using the macrophage marker Mac-3. (F) Quantification of necrotic core areas of aortic plaques. (G) Quantification of Masson's trichrome (collagen) staining of aortic plaques. (H-N) Therapeutic targeting of CD146 with antibody reduces atherosclerosis (five mice per group). All the quantifications were performed using at least 15 sections per mouse. (H) Intervention dosing regimen of CD146-targeted therapy using anti-CD146 AA98 or control IgG in HFD-induced atherosclerosis. ApoE−/− mice were fed a HFD for 12 weeks before antibody treatment for additional 6 weeks, the time at which the mice were sacrificed. (I) Lesion area of atherosclerotic plaques of the aortic roots of each group of mice, presented for each group across the 400 μm of the aortic root (n = 5). (J) Quantification of lesion area of aortic plaques isolated from ApoE−/− mice. (K) The distribution of early, moderate and advanced plaques based on histological staging of the atherosclerotic lesions. (L) Quantification of immunostained aortic plaques for macrophages using the macrophage marker Mac-3. (M) Quantification of necrotic core areas of aortic plaques. (N) Quantification of Masson Trichrome (collagen) staining of aortic plaques. The scale bar is 100 μm. One-way ANOVA test, *P< 0.05, **P< 0.01, ***P< 0.001.

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