Skip to main content
. 2016 Dec 9;27(2):226–240. doi: 10.1038/cr.2016.147

Figure 1.

Figure 1

HDP1 and HDP2 prevent transcriptional silencing of transgenes. (A) Identification of hdp1 and hdp2 mutants. Introduction of HDP1 and HDP2 genomic DNA fully complemented the root phenotype in hdp1-1 and hdp2-1 mutants. (B) RT-qPCR showing significantly reduced transcript levels of the transgenes SUC2 and NPTII in hdp1-1 and hdp2-1 mutants in comparison to 35S::SUC2 plants. HDP1 and HDP2 genomic DNA rescued the silencing of SUC2 transgene in hdp1-1 and hdp2-1 mutants. Two representative transgenic lines were selected and relative expression of SUC2 transcript was normalized to 35S::SUC2 plants. Three independent biological replicates were carried out for statistical analysis. See also Supplementary information, Figure S1. (C) Genomic structure and mutant alleles of HDP1 gene. For hdp1-1 and hdp1-2 mutants, nucleotide substitutions of “G” to “A” lead to the changes of 226th and 97th amino acids from tryptophan (W) to stop codons. hdp1-3 and hdp1-4 mutants are generated by CRISPR/Cas9-mediated genome editing. Nucleotide insertions of “A” and “T” were identified in hdp1-3 and hdp1-4 mutants, respectively. Red box indicates the coding region of AT1G72270 gene and gray box indicates untranslated regions. (D) Genomic structure and mutant alleles of HDP2 gene. For hdp2-1 mutant, a G-to-A substitution causes a pre-mature stop codon. An insertion of “T” was identified in hdp2-2 mutant. Blue box indicates the coding region of AT4G31270 gene and gray box indicates untranslated regions.