Fig. 2.

MT-binding and actin-binding activities of Shot are essential for efficient zippering of the dorsal hole in Drosophila embryos. (A) Zippering deffects of shot^sf20, shot^kakP1, shot^ΔEGC and shot^ΔEGC/shot^kakP1 mutant embryos at a closure stage of 30 µm hole width. Scale bar: 50 µm. (B) Length-to-width ratio of the dorsal opening when it is at a 30 µm width in various shot mutants. (C) Schematic representation of the longest available transgenic Shot protein version [Shot-L(A)–GFP] and its deletion constructs. *, mutated MtLS motifs. Rescue section: –, no rescue; +, rescue; a, for the lack of rescue, see explanation in the text. Localization section: –, no localization; +, weak localization; ++ strong localization; b, strong localization to the MT lattice may mask plus-tip localization; c, diffuse cytoplasmic localization may mask faint localization to the MTs. (D) Length-to-width ratio of the dorsal hole when it is at a 30 µm width in transgenic rescue experiments. Truncated versions of Shot-L(A)–GFP lacking various protein domains were expressed in the epithelia of shot^sf20 null mutant embryos. Mean±s.d. is shown for all quantitative data. ***P<0.001 (t-test).