Fig. 3.

Abnormal MTs in the shot^sf20mutant DME cells. (A,B) Immunofluorescence staining of wild-type (A) and shot^sf20 mutant (B) embryos at the dorsal closure stage. Tubulin staining labels the MT network (red); actin is labeled with phalloidin (green). White arrows indicate abnormally long and curled MTs at the leading edge of shot^sf20mutant DME cells. (C,D) Frames taken from movies showing Tubulin–EGFP-expressing DME cells in wild-type (C) and in shot^sf20mutant (D,E) embryos. White arrows indicate MTs growing into protrusions. MTs are abnormally long and curled at the leading edge of shot^sf20mutant DME cells. (D′,E′) Enlargement of the boxed regions in D and E showing bending of a MT in D′ and protrusion of a MT at the lateral surface in E′. (F,G) Staining for acetylated tubulin labels stabilized MTs (red); actin is labeled with phalloidin (green). In shot^sf20 mutants, no abnormal distribution of stabilized MTs is detectable. Scale bars: 5 µm (C,D,E); 10 µm (A,B,F,G).