Fig. 2.

Clasp2 expression in human keratinocytes prevents premature differentiation. (A) Clasp2 localization in human skin (arrowhead). Scale bars: 50 μm (left); 10 μm (enlarged area on right). (B) Immunoblot of Clasp2 in human keratinocytes (hKer). LC, low Ca²⁺. (C) Clasp2 mRNA levels in scramble and Clasp2 siRNA primary human keratinocytes. (D) mRNA levels of differentiation genes in scramble and Clasp2 siRNA primary human keratinocytes relative to levels of Gadph. Data are presented as mean±s.e.m. *P<0.02, ***P≤0.0006; ns, non-significant (Mann–Whitney U test); n=3–4 independent experiments per panel.