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. 2017 Mar 7;7:43375. doi: 10.1038/srep43375

Figure 3. Non-specific protein adsorption and selective cell capture.

Figure 3

(A) Protein-repulsive characteristics on oligopeptide SAMs. Bare gold surface (open column), the SAM surface (striped column), and the aptamer-conjugated SAM surface (solid column) were exposed to fibronectin and proteins in 10% FBS. The error bars indicate the standard deviations calculated from four independent experiments with QCM. (B,C) Hep G2 cells and RFP-HNDFCs captured on an aptamer-modified surface. Phase-contrast and fluorescent images were merged. Red fluorescence signal indicates RFP-HNDFCs. The two cell types were seeded at a ratio of 1:1 on the gold surfaces modified with (a) 3% aptamer/oligopeptide, or (b) without any modification. (D) Selective cell capture on SAMs modified with aptamer. A mixed suspension of Hep G2 cells (target, solid column) and HNDFCs (non-target, open column) was seeded on the aptamer-SAM surfaces of different aptamer densities. The relative density of aptamer represents the molar ratio of the aptamer to the oligopeptide calculated from QCM data. The error bars indicate the standard deviations calculated from nine independent experiments. *p < 0.05.