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. 2017 Mar 7;7:43485. doi: 10.1038/srep43485

Figure 6. Gene co-expression subnetwork of ENSMUST00000117266 and its role in cardiomyocyte proliferation.

Figure 6

(a) Co-expression subnetwork of ENSMUST00000117266. (b) Cardiomyocytes were cultured under normoxic or hypoxic conditions for 6 hours and the expression of ENSMUST00000117266 was determined by real time qRT-PCR. (c) 0.9% saline solution or paraquat (5 mg/kg) was daily administrated subcutaneously to neonatal mouse for 3 days after birth and the expression of ENSMUST00000117266 was determined in heart harvested at P3. (d) The expression of ENSMUST00000117266 at indicated time point after sham or MI surgery was determined by qRT-PCR. (e) The protein expression of LAMIN A and GAPDH in nucleus and cytoplasm of P1 mouse cardiomyocytes was analyzed by immunoblotting. The RNA levels of Gapdh and ENSMUST00000117266 in nucleus and cytoplasm of P1 mouse cardiomyocytes were analyzed by qRT-PCR. (f) Cell cycle analysis of primary mouse cardiomyocytes after ENSMUST00000117266 knockdown by using PI staining. Representative histograms and statistical data are shown. (g) The mRNA expressions of Hand1, Larp1 and Sap30l in primary mouse cardiomyocytes were assessed by qRT-PCR after 48-hours ENSMUST00000117266 siRNA (left) or ASO (right) transfection. Data are normalized to Gapdh. Data are shown as mean ± S.D. *p < 0.05, **p < 0.01 and ***p < 0.001 denote statistical comparison between the two marked groups, respectively. T approximation test for (b) unpaired two-tailed Student’ t-test for (d,f and g); paired two-tailed Student’ t-test for (c and e). (n = 3 cultures per group in (b and e) n = 3 samples per group in (c); n = 4 samples per group in (d); n = 4 cultures per group in (f); n = 5 cultures per group in (g).