Figure 4. Exon 9 and 8 alignment.

Alignment was performed using Ensemble (http://www.ensembl.org/index.html). In red are highlighted the common mismatches between human and mouse sequence; in green are highlighted the mismatches between all three organisms. The star (*) indicates conserved nucleotides. The sequence changes created separately in the human minigene for transforming it to a more mouse-like genotype are highlighted in blue. (A) Alignment of exon 9. The 3′ss, upstream human exon 9, is shown in bold. The human stop codon is underlined. (B) Alignment of exon 8 T. Part of the 3′ss upstream exon 8 T is shown in bold characters. Splicing regulatory proteins predicted with SpliceAid to bind exon 8 T are indicated.