Skip to main content
. 2004 Dec;24(24):10703–10717. doi: 10.1128/MCB.24.24.10703-10717.2004

FIG. 6.

FIG. 6.

7-Kchol induces Ca2+ oscillations in SMCs. Untransfected and Nox-4 siRNA-transfected SMCs were loaded with Fluo-3/AM (5 μM, 1 h, 37°C) and placed in a culture chamber adapter to an inverted microscope. Then, 40 μg of 7-Kchol or 7-ketocholesteryl-3-oleate/ml were added 3 min after the start of the experiment. The images were generated by confocal microscopy every 10 s over a period of 20 min. The data are reported as the fluorescence intensity, reflecting changes in [Ca2+]i. The upper panel provides an illustration of real-time imaging generated by confocal microscopy.