FIG. 7.

Silencing of Nox-4 expression prevents the UPR induced by 7-Kchol. (A) Lysates of SMCs transfected with scrambled or Nox-4 siRNAs incubated with or without 40 μg of 7-Kchol/ml for 24 h were immunoblotted for CHOP/GADD 153, GRP78/Bip, and proapoptotic Bax and for antiapoptotic Bcl-2 and α-actin. (B) The relative protein content of samples was determined with an anti-α-actin antibody. The bars represent the densitometric value for each experimental condition and are representative of three separate experiments.