FIG. 9.

IRE-1 controls the 7-Kchol-induced expression of Nox-4. SMCs were transfected with scrambled siRNA or IRE-1 siRNA duplexes. After incubation for 24 h in complete medium, SMCs were exposed to 0 or 40 μg of 7-Kchol/ml for 16 h. The IRE-1 protein was detected by Western blot analysis with the anti-IRE-1 antibody, which recognizes both the wild-type (IRE-1) and the phosphorylated forms (IRE-1-P) of IRE-1. Cells lysates were analyzed by Western blotting with antibodies directed against JNK phospho-specific Thr¹⁸³ and Tyr¹⁸⁵, Nox-4, and α-actin. The Western blots shown are representative of three separate experiments.