Table 1.
Primers used in Sanger sequencing performed in a patient with Muckle-Wells syndrome
| NLRP3 Exons | Region of Sequence | Primers | Sequences |
|---|---|---|---|
| Exon 1 | 2652-3256 | Ex1-F | 5’-CCGGCCTTGGCTAACTTTTC-3’ |
| Ex1-R | 5’-CCTGGGCAACAAGAGCAACT-3’ | ||
| Exon 2 | 7109-7532 | Ex2-F | 5’-GCAGAAATGCTCCCAACCAG-3’ |
| Ex2-R | 5’- TGGAATGCAAAGGCCTGTCT -3’ | ||
| Exon 3 | 7797-8522 | Ex3-1F | 5’-TTCTCCATTCCGGTTACCAC-3’ |
| Ex3-1R | 5’-AAGCAGCTTCTTTCTGATGAGG-3’ | ||
| 8231-9045 | Ex3-2F | 5’-GTCGGGGACACTCTACCAAG-3’ | |
| Ex3-2R | 5’-TCTCGCAGTCCACTTCCTTT-3’ | ||
| 8678-9512 | Ex3-3F | 5’-GGCAGCCTTCAGTCTGATTC-3’ | |
| Ex3-3R | 5’-ACTCCACCCGATGACAGTTC-3’ | ||
| 9292-9849 | Ex3-4F | 5’-GCAAGATCTCTCAGCAAATCA-3’ | |
| Ex3-4R | 5’-CCTCATCCCATTTATCCACCT-3’ |
Ex: Exon; F: Forward; R: Reverse; NLRP3: NOD-like receptor family pyrin domain-containing protein 3.