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. 2017 Mar 7;18:14. doi: 10.1186/s12865-017-0195-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of duration of antigen stimulation on IFN-γ ELISpot test results. SFC counts (means of four replicates) in IFN-γ ELISpot following stimulation of PBMC samples from three CMV-seropositive healthy donors (d120, 32-year-old male; d254, 62-year-old female; d270, 22-year-old female) with T-activated® IE-1 or pp65 for 17, 19 and 21 h. Unstimulated PBMC (neg.) were used as a negative control. Differences between stimulation durations were tested using the non-parametric two-sided One-way ANOVA Kruskal-Wallis test (*P < 0.05). P-values < 0.05 were considered statistically significant