Fig. 4.

Evaluation of different cell culture media on the performance of IFN-γ ELISpot. a Boxplot diagram showing ELISpot results upon stimulation of PBMC of 10 CMV-seropositive healthy blood donors (median age and range of 31 (26–54) years; 40% male and 60% female) with T-activated® pp65 in various cell culture media. Median values (horizontal black lines) are indicated in brackets. Differences between medium conditions among stimulated conditions were tested using the non-parametric two-sided One-way ANOVA Kruskal-Wallis test (p = 0.613). b ELISpot results of the non-stimulated cells incubated in AIM-V or in UltraCulture (UC) serum-free media are shown separately with an expanded Y-axis scale. Differences between both conditions were tested using the non-parametric two-sided MWU test (p = 0.074). P-values < 0.05 were considered statistically significant. Serum-containing media were composed of RPMI 1640 supplemented with 5% serum (R5): FCS, NTA, NTS or human AB (hAB)