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. 2017 Mar 7;18:14. doi: 10.1186/s12865-017-0195-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Comparison of IFN-γ ELISpot performance using different microtiter plates. ELISpot were performed using PBMC from three CMV-seropositive healthy donors (two preparations each, ELISpot in quadruplicate, ran twice) on various microtiter plate materials (MCE plate, PVDF plate, PVDF strips). Age (gender) of donors was 32 (male), 33 (male) and 51 (female). SFC values per 200,000 PBMC are shown as box plots for non-stimulated PBMC (neg.), and for T-activated® IE-1- and pp65-stimulated PBMC. Median values (horizontal black lines) are indicated in brackets. Differences between microtiter plate materials were tested using the non-parametric two-sided One-way ANOVA Kruskal-Wallis test (**P < 0.01; ***P < 0.001)