Fig. 6.

titration of the ifn-γ capture antibody. PBMC (four replicates each) from five CMV-seropositive healthy donors (median age and range of 31 (22–49) years; 1 male and 4 female) were seeded on PVDF microtiter strips coated with increasing concentrations (2.5 to 7.5 μg/ml) of IFN-γ capture antibody. Cells were left unstimulated (neg.) (a, b) or were stimulated with T-activated® IE-1 (a) or pp65 (b, c) CMV antigens, as before. SFC mean values per 200,000 PBMC are shown as box plots for the collective of 5 donors (median values indicated in brackets) (a, b) and as histograms (c) for pp65-stimulated PBMC of individuals donors (d032, d120, d172, d202, d241). Differences between coating conditions were tested using the non-parametric two-sided One-way ANOVA Kruskal-Wallis test (*P < 0.05; **P < 0.01)