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. 2017 Mar 7;18:14. doi: 10.1186/s12865-017-0195-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Assay validation in immunocompetent donors. PBMC isolated from whole blood of 45 healthy donors (Table 1) were assayed using the optimized IFN-γ ELISpot. A positivity cut-off of 10 SFC/200,000 PBMC (grey horizontal dashed line) was defined (see text and Additional File 2). Considering a test result as positive when the geometric mean for at least one of the IE-1 or pp65 stimulated approach is ≥ 10 SFC/200,000 PBMC and when the ratio of geometric means of stimulated to unstimulated conditions is ≥ 2.5, positive agreement (sensitivity) and negative agreement (specificity) of the optimized IFN-γ ELISpot test results with CMV serology within this collective of healthy donors was 97% and 85% respectively