Skip to main content
. 2004 Dec;24(24):10542–10557. doi: 10.1128/MCB.24.24.10542-10557.2004

FIG. 6.

FIG. 6.

Ligand and mitogen-induced PR transcriptional activity is enhanced in cells with low levels of p27. (A) Absence of CDK2-induced PR activity in p27-rich T47D cells. T47D-YB cells were transiently transfected with a PRE-luciferase reporter along with increasing concentrations of CDK2-TY. PRE-luciferase activity was measured and normalized to that of Renilla controls. Vector control cultures (vector) received 1.0 μg of parental vector control (for CDK2-TY). (B) p27 RNAi restores CDK2-induced PR transcriptional activity in T47D-YB cells. T47D-YB cells were transiently transfected with PRE-luciferase and Renilla reporter plasmids, CDK2-TY (+) or its parental control vector (−), and pSHAG vectors expressing either control RNAi (−) or p27 RNAi (+). Following treatment with R5020 (10 nM) for 18 h, PRE-luciferase activity was measured and normalized to that of Renilla controls. (Inset) p27 RNAi knock-down of p27. P27 RNAi or control RNAi was cotransfected into T47D-YB cells, and cell lysates were blotted for p27 and β-actin. The data are presented as means ± standard deviations of three replicates for each data point. Results are representative of two to three independent experiments.