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. 2017 Mar 7;17:175. doi: 10.1186/s12885-017-3166-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — miR-124 inhibits CCA cell invasion and metastasis. a The miR-124 levels in two CCA cell lines, QBC939 and RBE, were lower than those in cultured primary biliary epithelial cells, determined by real-time PCR analysis. b, c Validation of the miR-124 levels after transfection. d The effect of miR-124 overexpression on CCA cell invasion, as shown by Transwell assays in vitro. e The effect of miR-124 overexpression on CCA cell migration according to wound healing assays in vitro. f The effect of miR-124 overexpression on QBC939 cell metastasis following xenotransplantation into nude mice by intrasplenic injection. In-miR-124: CCA cells transfected with miR-124 inhibitors; Ex-miR-124: CCA cells transfected with miR-124 mimics. *P < 0.05