Fig. 3.

miR-124 downregulates GATA6 expression by directly targeting its 3′-UTR in CCA cells. a, b The effect of miR-124 overexpression and downregulation on GATA6 protein levels in CCA cells, determined by western blot analysis. c, d Correlation between GATA6 and miR-124 expression in 57 CCA samples. e Mutation of the potential binding site in the 3′-UTR of GATA6. f The effect of miR-124 on the relative luciferase activity of the GATA6 wild-type and mutant 3′-UTR constructs. In-miR-124: CCA cells transfected with miR-124 inhibitors; Ex-miR-124: CCA cells transfected with miR-124 mimics. *P < 0.05, **P < 0.01