. 2017 Mar 6;18:13. doi: 10.1186/s12865-017-0197-9

Table 1.

List of domain specific mutations in E protein of GI isolates in reference (Ref) to GIII strains

		Genotype I (GI)				Genotype III (GIII)
E Protein	Mutations	AB051292 [Japan, Ishikawa, 1994]	JN703381 [WB, JEV28 2010]	JN703382 [WB, JEV21 2010]	KC526872 [WB, JEV45 2011]	3P54	SA14 virulent M55506	SA14-14-2 Non-virulent JN604986
Domain I	N--2H	.	+	+	+	N	N	N
	N--2 T	+	.	.	.	N	N	N
	Y183F	+	.	.	.	Y	Y	Y
	R193K	+	.	.	.	R	R	K
	K138E^a	(+)^a	(+)^a	(+)^a	(+)^a	K^b	E	K
	V176I^a	(+)^a	(+)^a	(+)^a	(+)^a	V^b	I	V
	A177T^a	(+)^a	(+)^a	(+)^a	(+)^a	T	T	A
Domain II	C-60Y	+	.	.	.	C	C	C
	N103K	+	+	+	+	N	N	N
	F107L^a	(+)^a	(+)^a	(+)^a	(+)^a	F^b	L	F
	T129M	+	.	.	.	T	T	T
	A222S	+	+	+	+	A	A	A
	G244E	+	+	+	+	G	G	G
	G261S	.	+	+	+	G	G	G
	H264Q^a	(+)^a	(+)^a	(+)^a	(+)^a	Q	Q	H
	M279K^a	(+)^a	(+)^a	(+)^a	(+)^a	M^b	K	M
Domain III	V315A	+	+	+	+	V	V	V
	S327T	+	+	+	+	S	S	S
	A366S	+	.	.	.	A	A	A
	V372L	+	.	+	+	V	V	V
	M374I	+	.	+	+	M	M	M
	G388K	+	.	+	+	G	G	G
	W396R	+	.	+	+	W	W	W
Total mutations		21	13	17	17	4	6	Ref
Net mutations		15	7	11	11	-	-

Amino acids marked with ^bsign in 3P54 have been identified as mismatches between PDB and UNIPROT sequence (P27395)

All comparisons are made for first 406 residues of E protein (ecto domain). All three GI isolates of Midnapur/WB are taken with earlier Japan strain (Ishikawa). In GIII category, three sequences such as 3P54_A, virulent SA14 (ID M55506) and attenuated non-virulent SA14-14-2 (ID JN604986) are considered. + Sign indicates presence and dot indicates absence of mutations respectively. (+) indicates category of reported neurovirulent mutations [13]. Identical neurovirulent mutations of present isolates as SA14 are identified with^a sign