Table 3.
Methylome-CpGs associated with birth weight at a false discovery rate of 0.05
| CpG | CHR | POS | IQR | Est | 95% CI | P | Gene | Annotation |
|---|---|---|---|---|---|---|---|---|
| cg00510507 | 10 | 61900413 | 8.4 | 4.9 | (3.5 to 6.2) | 4.6 × 10–8 | ANK3 | 5’ UTR |
| cg08390209 | 9 | 22005563 | 6.6 | 7.1 | (5.1 to 9.0) | 4.9 × 10–8 | CDKN2B | 3’ UTR |
| cg23671997 | 15 | 65677753 | 4.6 | 9.2 | (6.5 to 12) | 1.6 × 10–7 | IGDCC4 | Intron |
| cg14300531 | 11 | 73969506 | 9.6 | –3.9 | (–5.0 to –2.8) | 4.0 × 10–7 | P4HA3 | Intron |
| cg25685359 | 22 | 46473721 | 8.8 | –3.7 | (–4.8 to –2.6) | 9.9 × 10–7 | MIRLET7BHG | Non-coding |
| cg22383874 | 17 | 48670670 | 4.8 | 7.6 | (5.2 to 10) | 1.2 × 10–6 | CACNA1G | Intron |
| cg02729344 | 16 | 49888237 | 6.6 | 6.8 | (4.7 to 9.0) | 1.9 × 10–6 | ZNF423 | Intron |
| cg25487405 | 22 | 46473039 | 5.5 | –5.6 | (–7.2 to –3.9) | 2.2 × 10–6 | MIRLET7BHG | Non-coding |
Eight CpGs were significantly associated with birth weight at a false discovery rate (FDR) of 0.05. The eight CpGs mapped to seven loci (two CpGs mapped to MIRLET7BHG). Regression coefficients (Est), 95% confidence intervals (CI) and P values are reported as percentage change in birth weight for 10% increase in percent methylation. Interquartile range (IQR), chromosome (CHR) and position (POS) of CpG are also shown. Analysis was done by linear regression of log-transformed birth weight against methylation at each CpG site, adjusted for child sex, gestational age, ethnicity, cellular proportions and interactions between ethnicity and cellular proportions