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. 2017 Mar 7;18:29. doi: 10.1186/s12868-017-0345-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Representative images showing cholinergic lesions after the injection of 192 IgG-saporin. a Atlas schematic showing the medial septum. The square indicates the location at which the images were taken. b The normal group exhibited a large number of choline acetyltransferase (ChAT)-immunopositive neurons (red) in the medial septum. The lesion groups (c) and all the stimulation groups (d pre-stimulation, e training-stimulation, f probe-stimulation), which were all injected with 192 IgG-saporin, exhibited a loss of ChAT-immunopositive neurons. g The number of ChAT-immunopositive neurons was significantly reduced by 192 IgG-saporin (p < 0.05)