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. 2017 Mar 7;17:140. doi: 10.1186/s12906-017-1655-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Identification of Rhein in the brain tissue of a CCI rat by Ultra-high Performance Liquid Chromatography with tandem mass spectrometry (UPLC-MS/MS) analysis. Representative MRM chromatograms of Rhein in brain sample. a Brain sample detection at 1 h after intragastric administration of Rhubarb (12 g/kg); (b) Blank brain tissue spiked with reference Rhein