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. 2017 Mar 6;14:48. doi: 10.1186/s12974-017-0814-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — No overt difference in expression of C1q in liver cells and kidney macrophages in C1qa ^FL/FL:Cx3cr1 ^CreERT2 compared to wild type mice, correlating with lack of YFP/Cre expression in these tissues in C1qa ^FL/FL:Cx3cr1 ^CreERT2. C1q staining of the liver (a) or kidney (b) WT (top panels), C1qa ^FL/FL :Cx3cr1 ^CreERT2 (middle panels). C1q reactivity is absent from C1q^GT/GT (bottom panels) as expected demonstrating specificity of the antibody in these tissues. Representative pictures of n = 2–5 mice per genotype per age at 4–6 months. C1q (red) but not YFP (green) is detected in the liver (c) and kidney (d) of Cx3cr1 ^CreERT2 mice (4 months). Bottom panels of (c and d) are the merged images of C1q and YFP. Scale bars: 50 um