Table 1.
Effect of Promoter Specific CreERT2 Expression
| Model | Target Gene | Cre construct | Tamoxifena | Effect on target gene expressionb | |||||
|---|---|---|---|---|---|---|---|---|---|
| IHC | Western blot | QPCR | Titer | ||||||
| Brain | Liver Kidney | Brain | Blood | Brain | Blood | ||||
| C57Bl6J | C1qa | Thy1 CreERT2 | - | - | - | - | - | ||
| + | - | - | - | - | |||||
| C57Bl6J | C1qa | Cx3cr1 CreERT2/WganJ | - | ↓ | - | ↓ | - | ↓ | - |
| + | ↓ | - | ↓ | - | ↓ | - | |||
| C57Bl6J | C1qa | Rosa26 CreERT2 | - | - | - | ||||
| + | ↓ | ↓ | |||||||
| Arctic | C1qa | Cx3cr1 CreERT2/WganJ | - | ↓ | ↓ | - | ↓ | ||
| C57Bl6J | CAG-tdTomato | Cx3cr1 CreERT2/WganJ | - | ↑c | |||||
a"-" indicates no treatment, "+" indicates Tamoxifen treatment as described in Materials and Methods
b"-" indicates no change from wild type control and arrow down indicates decreased C1q
cevidence of nuclear recombinase activity