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. 2017 Mar 6;18:3. doi: 10.1186/s12858-017-0078-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Kinetics of cross-linking of Fl-histatin-3 and Fl-histatin-5 to G- and F-actin and effect of phalloidin on the extent of cross-linking of Fl-histatin-3, Fl-histatin-5 and Fl-LL-37 to F-actin. a Kinetics of cross-linking of 6 μM Fl-histatin-3 and −5 to 4 μM F-and G-actin. Samples were treated with 0.3 mg/ml TGase at 22 °C for various time intervals, run on SDS-PAGE and evaluated by densitometry. b Effect of 8 μM phalloidin on the extent of cross-linking of 8 μM Fl-histatin-3, Fl-histatin-5 and Fl-LL-37 to 4 μM F-actin. Cross-linking with 0.3 mg/ml TGase for 30 min at 22 °C. Upper panel, quantitative evaluation of SDS-PAGE by densitometry. Lower panel Coomassie blue stained representative SDS-PAGE