Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 7;8:370. doi: 10.3389/fmicb.2017.00370

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Ma, Liu, Li, Fu, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Identification of ΔNcROP5 complementary strain, iΔNcROP5. (A) RT-PCR analysis of NcROP5 gene transcription. (B) Western blotting analysis of Nc-1, ΔNcROP5 and iΔNcROP5 tachyzoites. The expected band was detected by the anti-HA monoclonal antibody and anti-rNcROP5 polyclonal antibody. NcActin was used as the control. (C) IFA analysis of iΔNcROP5 tachyzoites. The HA-tagged ROP5 and ROP5 proteins (green) were localized at the anterior end of the parasites, and HA co-localized with ROP5. The shapes of parasites were stained with anti-NcSRS2 (red), and the nuclear DNA was stained with Hoechst (blue). Scale bar, 1.0 μm.