Figure 5.

Integrin blocking reduces cation-dependent adhesion strength in nonmalignant cells. (A) MCF10A cells were stained for paxillin (green) and nuclei (blue). Open and closed arrowheads indicate FAs for the indicated RGD culture condition. Scale bars, 100 μm. (B) Plot of the number of FAs per cell area for cells without (black) and with (red) RGD. (C) Normalized cell density is plotted versus shear stress for cells without (black) and with (red) RGD. The inset shows the average τ₂₅ adhesion strength for each condition in dynes per square centimeter. ^∗p < 0.05. All dot plots represent triplicate experiments analyzing >20 cells per condition. Shear plots represent binned averages from biological triplicate experiments performed across multiple, overlapping shear ranges. All adhesion-strength assays were performed using fibronectin-coated coverslips. (D) Plot of cell velocity, in micrometers per hour, for cells treated (red) or not treated (black) with RGD on fibronectin-coated coverslips. ^∗∗∗p < 0.001 for comparisons with unpaired t-test with Welch’s correction. To see this figure in color, go online.