Figure 4. Prolonged TNFα stimulation suppresses AICAR dependent AMPK activation.
A. HEK293T cells were incubated with TNFα (10 ng/ml) for 12, 24 or 36 h and then stimulated with 2 mM AICAR for 10 min. Immunoblots were performed with the indicated antibodies. These are representative images from 3 independent experiments. B., C. Signal quantifications of expression levels of phospho- T172 AMPKα corrected by total AMPKα (B) and levels of phospho- S79 of ACC to total ACC (C). The data are presented as mean ± s.e.m. Statistical examination between control and AICAR stimulated cells in each sets were performed. **p < 0.01, ***p < 0.005.