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. 2016 Sep 8;7(46):74612–74629. doi: 10.18632/oncotarget.11916

Figure 5. Prolonged TNFα stimulation up-regulates Fyn activity in HEK293T cells.

Figure 5

A. Fyn kinase activity in HEK293T cells treated or not with TNFα (10ng/ml) for 36 h. These are representative images from 5 independent experiments ***p < 0.005. B. Fyn expression in HEK293T cells treated or not with TNFα for 36 h. C. After a 36-h incubation with 10ng/ml TNFα, HEK293T cells were treated or not with TNFα for 36 h and homogenized. Immunoprecipitation was performed with total AMPKα antibody followed by immunoblotting with the 4G10 and total AMPKα antibody. These are representative images from 4 independent experiments. D. Signal quantification (Y436 normalized to total AMPKα) from (C). (***P < 0.005).