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Validation of SPICER using experimentally acquired 2d SMT data of RNAP in live E. coli cells. (a and b) Comparison of the identified D1 and D2 (a) and and (b) values using SPICER, 1d, and 2d analyses. (c and d) Simulation of a similar system showing the same trend, that 2d and SPICER analyses are significantly more accurate than the 1d analysis, with SPICER reflecting the true values most closely. The true values for the simulation are shown as horizontal black lines.
