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. 2016 Oct 21;7(48):78713–78725. doi: 10.18632/oncotarget.12805

Figure 2. Morphological differences following knockdown of SIN3 paralogs in metastatic breast cancer cells.

Figure 2

(A, B) MDA-MB-231 and (C, D) MDA-MB-435 cells stably transduced with SIN3A or SIN3B targeting shRNA (see Supplementary Table S1 for sequences) were seeded on 96-well plates. Proliferation was assessed at times indicated using Alamar Blue reagent. No statistically significant differences were noted in the doubling times. (E) Cells were seeded on 24-well plates coated with Matrigel and incubated at 37°C for 10 days. Three representative images for MDA-MB-231 cells are shown. The sh3 construct was used for SIN3A and sh2 for SIN3B. Dual knockdown used both sh3 for SIN3A and sh2 for SIN3B as described in the Materials and Methods. A more invasive phenotype was noted for the SIN3A knockdown and epithelial-like phenotype noted for the SIN3B knockdown. Scale bar is 50 μm. (F) The number of invasive colonies (defined by colonies with spiculations) for each cell line was quantified and normalized to the vector control. *indicates p < 0.05 compared to vector control.